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刘林

 性    别:男
 职    称:教授
 所属部门:遗传学和细胞生物学系
 招生专业:细胞生物学
 研究方向:干细胞与发育生物学
::教师简介::
刘林,博士,南开大学生命科学学院,南开大学杰出教授。教育部“长江学者”奖励计划特聘教授,国家杰出青年基金获得者。主持了多项国家级的重大项目,包括国家重大科学研究计划(973)、国家重大转基因专项、国家国际科技合作专项等科研基金的资助。曾获得多项奖励,包括2003年度美国生殖医学会辅助生殖技术奖(第一获奖人)和2010教育部高等学校科技进步二等奖。担任多个国际科技期刊编委和审稿人。在包括Nature Cell Biology、Nature Biotechnology、Nature、PNAS、Cell Research、EMBO Reports、Stem Cells、Human Molecular Genetics等SCI杂志发表论文100余篇。主要研究方向为端粒及再生在干细胞中的作用及表观遗传调控机制,以及卵巢衰老及抗衰老。

Tel: 86 22 23500752
Fax : 86 22 23500752        
Email: liutelom@yahoo.com; liulin@nankai.edu.cn
Website: http://stemcells-liu.org

研究领域: 
1. 端粒在体细胞重编程及在多能性干细胞多能性、肿瘤发生和复发中的作用及调节机制
2. 卵巢衰老机制及抗卵巢衰老的新策略

代表性论文:
1.	Yang J, Guo R, Wang H, Ye X, Zhou Z, Dan J, Wang H, Gong P, Deng W, Yin Y, Mao S, Wang L, Ding J, Li J, Keefe DL, Dawlaty MM, Wang J, Xu G, Liu L. Tet enzymes regulate telomere maintenance and chromosomal stability of mouse ESCs. Cell Reports 15:1809-1821 (2016).
2.	Zhang H, Panula S, Petropoulos S, Edsg?rd D, Busayavalasa K, Liu L, Li X, Risal S, Shen Y, Shao J, Liu M, Li S, Zhang D, Zhang X, Gerner RR, Sheikhi M, Damdimopoulou P, Sandberg R, Douagi I, Gustafsson J?, Liu L, Lanner F, Hovatta O, Liu K. Adult human and mouse ovaries lack DDX4-expressing functional oogonial stem cells. Nature Medicine 21:1116-1118 (2015).
3.	Chen H, Guo R, Zhang Q, Guo H, Yang M, Wu Z, Gao S, Liu L, Chen L. Erk signaling is indispensable for genomic stability and self-renewal of mouse embryonic stem cells. Proc Natl Acad Sci USA. 112: E5936–E5943 (2015).
4.	Sung LY, Chang WF, Zhang Q, Liu CC, Liou JY, Chang CC, Ou-Yang H, Guo RP, Fu HF, TK Cheng, Ding ST, Chen CM, Okuka M, Keefe DL, Chen YE, Liu L, Xu J. Telomere elongation and na?ve pluripotent stem cells achieved from telomerase haplo-insufficient cells by somatic cell nuclear transfer. Cell Reports 9:1603–1609 (2014).
5.	Dan J, Y Liu, N Liu, M Chiourea, M Okuka, T Wu, X Ye,C Mou, LWang, LL Wang, Y Yin, J Yuan, B Zuo, F Wang, Z Li, XPan, ZYin, L Chen, DL Keefe,S Gagos, A Xiao, Liu L. Rif1 maintains telomere length homeostasis of ES cells by mediating heterochromatin silencing. Developmental Cell 29: 7–19 (2014).
6.	Yuan J, Zhang D, Wang L, Liu M, Mao J, Yin Y, Ye X, Liu N, Han J, Gao Y, Cheng T, Keefe DL, Liu L. No evidence for neo-oogenesis may link to ovarian senescence in adult monkey. Stem Cells 31:2538-2550 (2013). 
7.	Wang F, Pan X, Kalmbach K, Seth-Smith ML, Ye X, Antumes DM, Yin Y, Liu L, Keefe DL, Weissman SM. Robust measurement of telomere length in single cells. Proc Natl Acad Sci USA. 110:E1906-1912 (2013).
8.	Jiang J, Lv W, Ye X, Wang L, Zhang M, Yang H, Okuka M, Zhou C, Zhang X, Liu L, Li J. Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Research 23:92-106 (2013).
9.	Liu M, Yin Y, Ye X, Zeng M, Zhao Q, Keefe DL, Liu L. Resveratrol protects against age-associated infertility in mice. Human Reproduction 28:707-717 (2013).
10.	Wang F, Yin Y, Ye X, Liu K, Zhu H, Wang L, Chiourea M, Okuka M, Ji G, Dan J, Zuo B, Li M, Zhang Q, Liu N, Chen L, Pan X, Gagos S, Keefe DL, Liu L. Molecular insights into the heterogeneity of telomere reprogramming in induced pluripotent stem cells. Cell Research 22:757-768 (2012).
11.	Huang J, Wang F, Okuka M, Liu N, Ji G, Ye X, Zuo B, Li M, Liang P, Ge WW, Tsibris JC, Keefe DL, Liu L. Association of telomere length with authentic pluripotency of ES/iPS cells. Cell Research 21:779-792 (2011).
12.	Agarwal S, Loh YH, McLoughlin EM, Huang J, Park IH, Miller JD, Huo H, Okuka M, Dos Reis RM, Loewer S, Ng HH, Keefe DL, Goldman FD, Klingelhutz AJ, Liu L, Daley GQ. Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients. Nature 464:292-296 (2010). 
13.	Huang J, Okuka M, Wang F, Zuo B, Liang P, Kalmbach K, Liu L, Keefe DL. Generation of pluripotent stem cells from eggs of aging mice. Aging Cell 9:113-125  (2010).
14.	Richards T, Wang F, Liu L, Baltz JM. Rescue of postcompaction-stage mouse embryo development from hypertonicity by amino acid transporter substrates that may function as organic osmolytes. Biology of Reproduction 82:769-777 (2010)
15.	Li C, Chen Z, Liu Z, Huang J, Zhang W, Zhou L, Keefe DL, Liu L. Correlation of expression and methylation of imprinted genes with pluripotency of parthenogenetic embryonic stem cells. Human Molecular Genetics 18:2177-2187 (2009). 
16.	Liu N, Lu M, Feng XM, Ma FX, Fang ZH, Tian XM, Ren Q, Zhang L, Liu B, Huang PP, Liu L, Han ZC. Exogenous Nanog alleviates but is insufficient to reverse embryonic stem cells differentiation induced by PI3K signaling inhibition. Journal of Cellular Biochemistry 106:1041-1047 (2009).
17.	Chen Z, Liu Z, Huang J, Amano T, Li C, Cao S, Wu C, Liu B, Zhou L, Carter MG, Keefe DL, Yang X, Liu L. Birth of Parthenote mice directly from parthenogenetic embryonic stem cells. Stem Cells  27:2136-2145 (2009).
18.	Keefe DL, Liu L. Telomeres and reproductive aging. Reproduction, Fertility and Development 21:10-14 (2009). 
19.	Cao S, Wang F, Chen Z, Liu Z, Mei C, Wu H, Huang J, Li C, Zhou L, Liu L. Isolation and culture of primary bovine embryonic stem cell colonies by a novel method. Journal of Experimental Zoology Part A Ecol Genet Physiol. 311A:368-376 (2009).
20.	Chen L, Liu L. Current progress and prospects of induced pluripotent stem cells.
        Sci China C Life Sci. 52:622-636  (2009).
21.	Huang J, Okuka M, McLean M, Keefe DL, Liu L. Effects of cigarette smoke on fertilization and embryo development in vivo. Fertility and Sterility 92:1456-1465 (2009).
22.	Liu L, Keefe DL. Defective cohesin is associated with age-dependent misaligned chromosomes in oocytes. Reprod Biomed Online 16:103-112 (2008).
23.	Huang J, Deng K, Wu H, Liu Z, Chen Z, Cao S, Zhou L, Ye X, Keefe DL, Liu L. Efficient production of mice from embryonic stem cells injected into four- or eight-cell embryos by piezo micromanipulation. Stem Cells  26:1883-1890 (2008)
24.	Liu L, S. M. Bailey, M. Okuka, P. Mu?oz, C. Li, L. Zhou, C. Wu, E. Czerwiec, L. Sandler, A. Seyfang, M. A. Blasco and Keefe. D. L. Telomere lengthening early in development. Nature Cell Biology 9: 1436-1441 (2007)
25.	Liu Y, Wu C, Lyu Q, Yang D, Albertini DF, Keefe DL, Liu L. Germline stem cells and neo-oogenesis in the adult human ovary. Developmental Biology 306:112-120 (2007)
26.	Liu L and Keefe DL. Chapter 15 Nuclear Transfer Methods to Study Aging in Methods in Molecular Biology: Biological Aging: Methods and Protocols. Humana Press Inc., Totowa, NJ. Pp191-207 (2007) 
27.	Keefe DL, Liu L, Marquard K. Telomeres and aging-related meiotic dysfunction in women. Cellular and Molecular Life Sciences 64:139-143 (2007) 
28.	Wang X, Liu L, Montagna C, Ried T, Deng CX. Haploinsufficiency of Parp1 accelerates Brca1-associated centrosome amplification, telomere shortening, genetic instability, apoptosis, and embryonic lethality. Cell Death & Differentiation 14:924-931 (2007)
29.	Keefe, D.L., Marquard, K., and Liu, L. The telomere theory of reproductive senescence in women. Current Opinion in Obstetrics and Gynecology 18:280-285 (2006).
30.	Dai, Y., L. Wang, H. Wang, Y. Liu, Q. Lyu, N. Li, D.L. Keefe, D.F. Albertini and L. Liu. Abnormalities in centrosome remodeling following somatic cell nuclear transfer (SCNT) in bovine oocytes. Reproduction 131:1051-1061 (2006).
31.	Navarro, P.A., Liu, L., Ferriani, R.A., and Keefe, D.L. Arsenite induces aberrations in meiosis that can be prevented by coadministration of N-acetylcysteine in mice. Fertility and Sterility 85: 1187-1194 (2006).
32.	Navarro, P.A., Liu, L., Trimarchi, J.R., Ferriani, R.A., and Keefe, D.L. Noninvasive imaging of spindle dynamics during mammalian oocyte activation. Fertility and Sterility 83:1197-1205 (2005). 
33.	Keefe, DL, Franco, S, Liu, L., Trimarchi, J, Cao, B, Weitzen, S, Agarwal, S, Blasco, MA. Telomere length predicts embryo fragmentation after in vitro fertilization in women--toward a telomere theory of reproductive aging in women. American Journal of Obstetrics and Gynecology 192(4):1256-60; discussion 1260-1(2005). 
34.	Liu, L., Franco, S., Spyropoulos, B., Moens, P.B., Blasco, M.A., and Keefe, D.L. Irregular telomeres impair meiotic synapsis and recombination in mice. Proc Natl Acad Sci U S A 101: 6496-6501 (2004)
35.	Liu, L., E. Czerwiec and D. L. Keefe. Effect of ploidy and parental genome composition on expression of Oct-4 protein in mouse embryos. Gene Expression Patterns 4: 433-441 (2004) 
36.	Liu, L., C.M. DiGirolamo, P.A.A.S. Navarro, M.A. Blasco and D. L. Keefe. Telomerase deficiency impairs differentiation of mesenchymal stem cells. Experimental Cell Research 294: 1-8 (2004)
37.	Navarro, P., Liu, L. and D.L. Keefe. In vivo effects of arsenite on meiosis and early embryo development in mice. Biology of Reproduction 70:980-985 (2004)
38.	Liu, L., J.R. Trimarchi, P. Navarro, M.A. Blasco and D.L. Keefe. Oxidative stress contributes to arsenic-induced telomere attrition, chromosome instability, and apoptosis. The Journal of Biological Chemistry 278:31998–32004 (2003)
39.	Keefe, D.L., Liu, L., W. Wang and C. Silva. Imaging meiotic spindles by polarization light microscope: principles and applications to IVF. Reproductive Biomedicine Online 7:24-29 (2003) 
40.	Liu, L. and D.L. Keefe. Ageing-associated aberration in meiosis of oocytes from Senescence-Accelerated Mouse (SAM). Human Reproduction 17: 2678-2685 (2002) 
41.	Liu, L., M.A. Blasco, J.R. Trimarchi and D.L. Keefe. An essential role for functional telomeres in mouse germ cells during fertilization and early development. Developmental Biology 249:74-84 (2002)
42.	Liu, L., J.R. Trimarchi, P. Smith and D.L. Keefe. Checkpoint for DNA integrity at the first mitosis after oocyte activation. Molecular Reproduction and Development 62:277-288 (2002)   
43.	Liu, L., M.A. Blasco and D.L. Keefe. Requirement of functional telomeres for metaphase chromosome alignments and integrity of meiotic spindles. EMBO Reports 3:230-234 (2002)
44.	Liu, L., M. Deng, X.C. Tian, and X. Yang. Chapter 17: Activation of mammalian oocytes: Principles and Practice. In: Introduction to Mammalian Reproduction. Editor: Daulat R.P. Tulsiani, Kluwer academic Publishers, MA, USA. pp-319-445 (2002)
45.	Liu, L., J.R. Trimarchi, P. Smith and D.L. Keefe. Mitochondrial dysfunction leads to telomere attrition and genomic instability. Aging Cell 1:40-46 (2002)
46.	Liu, L., James R. Trimarchi, Peter Smith and David L. Keefe. Haploidy but not parthenogenetic activation leads to increased incidence of apoptosis in mouse embryos. Biology of Reproduction 66:204-210 (2002)
47.	Trimarchi, J. R., Liu, L., Smith, P.J., and Keefe, D.L. Apoptosis recruits two-pore domain potassium channels used for homeostatic volume regulation. American Journal of Physiology 282:C588-594 (2002)
48.	Liu, L., Katherine Hammar, Peter J.S. Smith, Shinya Inoue, David L. Keefe. Mitochondrial modulation of calcium signaling at the initiation of development. Cell Calcium 30: 423-433 (2001)
49.	Dinnyés, A., Dai, Y., Barber, M., Liu, L., Xu, J., Zhou, P., and Yang, X. Development of cloned embryos from adult rabbit fibroblasts: effect of activation treatment and donor cell preparation. Biology of Reproduction 64: 257-263 (2001)
50.	Liu, L. Cloning efficiency and differentiation. Nature Biotechnology 19:406 (2001) (Correspondence).
51.	Liu, L., Trimarchi, J.R., Oldenbourg, R., and Keefe, D.L. Increased birefringence in the meiotic spindle provides a new marker for the onset of activation in living oocytes. Biology of Reproduction 63: 251-258 (2000)
52.	Trimarchi, J. R., Liu, L., Smith, P.J.S. and Keefe, D. L. Non-invasive measurement of potassium efflux as an early indicator of cell death in mouse embryos. Biology of Reproduction 63: 851-857 (2000)
53.	Trimarchi, J. R., Liu, L., Porterfield, M., Smith, P.J.S. and Keefe, D. L.  A non-invasive method for measuring preimplantation embryo physiology. Zygote 8: 15-24 (2000)
54.	Liu, L., Oldenbourg, R., Trimarchi, J.R., and Keefe, D.L. A reliable, noninvasive technique for spindle imaging and enucleation of mammalian oocytes. Nature Biotechnology 18: 223 – 225 (2000)

::主要学术论文::
::主要科研项目::