Longjiafu

Following finishing his undergraduate studies in Nankai University in 1999, China, Jiafu Long received his PhD degree of Biochemistry in 2004 for the works on the supramodular nature of neuronal scaffolding proteins in Mingjie Zhang’s lab (Hong Kong University of Science and Technology, Hong Kong) and subsequently carried out postdoctoral research in the same lab for three years. Then he begun independent research in Nankai University in 2007 as a specially-appointed Professor. In 2007, he was selected in the New Century Talent of the Ministry of Education of China, and he was one of the winners in the thirteenth Tianjin Youth Science and Technology Award in 2015. He is currently a Professor of Biochemistry and Molecular Biology in the College of Life Science and the State Key Laboratory of Medicinal Chemical Biology at Nankai University.

1995-1999 B.S. in Biochemistry and Molecular Biology, Nankai University, Tianjin, China 1999-2004 Ph.D. in Biochemistry, Hong Kong University of Science and Technology, Hong Kong, China

2004-2006, Postdoctoral Fellow, Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong 2006- present, Professor, College of Life Sciences and State Key Laboratory of Medicinal Chemical Biology, Nankai University 2020-2010， Visiting Professor, Weill Inistitute for Cell and Molecular Biology, Cornell University

Research in Long laboratory has focused on epigenetic gene regulation and disease. Currently, a series of interesting results have been obtained regarding the molecular mechanism of histone modification complex assembly and the regulatory mechanism of chromatin remodeling. From the angle of biochemistry and structural biology, these results promoted the epigenetic mechanism of multi-protein complexes involved in the regulation of gene expression, and make some contribution to the development of science.He has published original research articles in Nat Commun, Nat Struc Mol Biol, PNAS, Angew Chem Int Ed, Nucleic Acid Res, Cell Discov, Structure, FASEB J, JBC and among others.

SELECTED PUBLICATIONS: (listed the first or corresponding author only)

1.“Supramodular structure and synergistic target binding of the N-terminal tandem PDZ domains of PSD-95”, Long, J., Tochio, H., Wang, P., Fan J.-S,. Sala, C., niethammer, M., Sheng, M. and Zhang, M., J. Mol. Biol., 327(1): 203-214 (2003).

2.“The tetrameric L27 domain complex as an organization platform for supramolecular assemblies”, Feng, W.^&,Long, J.^&(equal contribution), Fan, J,-S., Suetake, T., Zhang, M., Nat. Struct. Mol. Biol., 11(5):475-480 (2004).

3． “A unified assembly mode revealed by the structures of tetrameric L27 domain complexes formed by mLin-2/mLin-7 and Patj/Pals1 scaffold proteins”, Feng, W.^&, Long, J.^&(equal contribution), Zhang, M.,Proc. Natl. Acad. Sci. USA, 102(19): 6861-6866 (2005).

4.“Autoinhibition of X11/Mint Scaffold Proteins Revealed by the Closed Conformation of the PDZ Tandem”, Long, J., Feng, W., Wang, R., Chan, L.-N., Fanny, Ip. Xia, J., Nancy, Ip. Zhang, M., Nat. Struct. Mol. Biol., 12(8): 722-728 (2005).

5.“Supramodular nature of GRIP1 revealed by the structure of its PDZ12 tandem in complex with the carboxyl tail of Fras1”, Long, J., Wei, Z., Feng, W., Yu, C., Zhao, Y. –X., Zhang, M., J. Mol. Biol., 375(5):1457-1468 (2008).

6.“Structural basis of b-catenin Recognition by Tax-interaction Protein-1”, Zhang, J., Yan, X., Shi, C., Yang, X., Guo, Y., Tian, C., Long, J.*, Shen, Y.*, J. Mol. Biol., 384(1): 255-263 (2008). (*: Co-corresponding author).

7. “Molecular Mechanism of Inward Rectifier Potassium Channel 2.3 Regulation by Tax-interacting Protein-1”, Yan, X., Zhou, H., Zhang, J., Shi, C., Xie, X., Wu, Y., Tian, C., Shen, Y.*, Long, J.*, J. Mol. Biol., 392(4): 967-976 (2009).

8. “Structural basis for tandem L27 domain-mediated polymerization”, Yang, X., Xie, X., Chen, L., Zhou, H., Wang, Z., Zhao, W., Tian, R., Zhang, R., Tian, C., Long. J.*, Shen, Y.*, FASEB J., 24(12): 4806-4815 (2010).

9. “The structural basis for the oligomerization of the N-terminal domain of SATB1”, Wang, Z., Yang, X., Chu, X., Zhang, J., Zhou, H., Shen, Y.*, Long, J.*, Nucleic Acids Res., 40(9): 4193-4202 (2012).

10. “Structure of an L27 domain heterotrimer from the cell polarity complex Patj/Pals1/Mals2 reveals a mutually independent L27 domain assembly mode”, Zhang, J., Yang, X., Wang, Z., Zhou, H., Xie, X., Shen, Y.*, Long, J.*, J. Biol. Chem., 287(14): 11132-11140 (2012).

11. “Rational design of a tetrameric Protein to enhance interactions between self-assembled fibers confers molecular hydrogels”, Zhang, X., Chu, X., Wang, L., Wang, H., Liang, G., Zhang, J., Long, J.*, Yang, Z.*, Angew. Chem. Int. Ed., 51(18): 4388-4392 (2012).

12. “Crystal structure of Elongator subcomplex Elp4-6”, Lin, Z., Zhao, W., Diao, W., Xie, X., Wang, Z., Zhang, J., Shen, Y.*, Long, J.*, J. Biol. Chem.287(25): 21501-21508 (2012).

13. “Open-closed mechanism of Mint2 regulates APP metabolism”, Xie, X., Yan, X., Wang, Z., Zhou, H., Diao, W., Zhou, W., Long, J.*, Shen, Y.*, J. Mol. Cell Biol.5(1): 48-56 (2013).

14. “Multifunctional biohybrid hydrogels for cell culture and cotrolled drug release”, Wang, H., Han, A., Cai, Y., Xie, Y., Zhou, H.*, Long, J.*, Yang, Z.*, Chem. Commun., 49(67): 7448-7450 (2013).

15. “Growth stimulation of bacterium Delftia by a peptide hydrogel”, Du, X., Wu, Z.,Long, J.*, Wang, L.*, RSC Adv., 3(40): 18259-18262 (2013).

16. “Structural insights into Paf1 complex assembly and histone binding”, Chu, X., Qin, X., Xu, H., Li, L., Wang, Z., Li, F., Xie, X., Zhou, H., Shen Y.*, Long, J.*, Nucleic Acids Res., 41(22): 10619-10629 (2013).

17. “Rational design of multifunctional hetero-hexameric proteins for hydrogel formation and controlled delivery of bioactive molecules”, Zhang, X., Zhou, H., Xie, Y., Ren, C., Ding, D.*, Long, J.*, Yang, Z.*, Adv. Healthc. Mater., 3(11): 1804-1811 (2014).

18. “Crystal structure of the Ubiquitin-like domain-CUT repeat-like tandem of special AT-rich sequence binding protein 1 (SATB1) reveals a coordinating DNA-binding mechanism”, Wang, Z., Yang, X., Guo, S., Yang, Y., Su, X-C., Shen, Y.*, Long, J.*, J. Biol. Chem., 289(40): 27376-27385 (2014).

19. “A genetically modified protein-based hydrogel for 3D culture of AD293 cells”, Du, X., Wang, J., Diao, W., Wang, L., Long, J.*, Zhou, H.*, PLoS One, 9(9): e107949 (2014).

20. “The Elp2 subunit is essential for Elongator assembly and functional regulation”, Dong, C., Lin, Z., Diao, W., Li, D., Chu, X., Wang, Z., Zhou, H., Xie, Z., Shen, Y.*, Long, J.*, Structure, 23(6): 1078-1086(2015).

21. “Angiomotin binding-induced activation of Merlin/NF2 in the hippo signaling pathway”, Li, Y., Zhou, H., Li, F., Chan, S., Lin, Z., Wei, Z., Yang, Z., Guo, F., Lim, C., Xing, W., Shen, Y., Hong, W., Long, J.*, Zhang, M.*, Cell Res., 25(7): 801-817 (2015).

22. “Dimerization of elongator protein 1 is essential for Elongator complex assembly”, Xu, H., Lin, Z., Li, F., Diao, W., Dong, C., Zhou, H., Xie, X., Wang, Z., Shen, Y.*, Long, J.*, Proc. Natl. Acad. Sci. USA, 112(34): 10697-10702 (2015).

23. “Self-assembly molecular chaperone to concurrently inhibit the production and aggregation of amyloid b peptide associated with Alzheimer's disease”, Hang, F., Qu A., Yang H., Zhu L., Zhou, H., Liu J., Long J.*, and Shi L.*, Acs Macro lett., 7: 983-989 (2018).

24. “Paf1  and Ctr9 subcomplex formation is essential for Paf1 complex assembly  and functional regulation”, Xie Y., Zheng M., Chu X., Chen Y., Xu H.,  Wang J., Zhou, H.*, Long J.*, Nat. Commun., 9(1): 3795 (2018).

25.  “ Treatment with Humanized Selective CD19CAR-T Cells Shows Efficacy in  Highly Treated B-ALL Patients Who Have Relapsed after Receiving  Murine-Based CD19CAR-T Therapies”, Zhao Y., Liu Z., Wang X., Wu H.,  Zhang J., Yang J., Zhang F., Liu L.,Long J.*, Lu P.*, Chen Z.*, Clin Cancer Res., 25(18): 5595-5607 (2019).

26. “A  Heterotrimeric SMARCB1-SMARCC2 Subcomplex is Required for the Assembly  and Tumor Suppression Function of the BAF Chromatin-remodeling Complex”, Chen, G., Zhou, H.*, Liu, B., Wang, Y., Zhao, J., Giancotti, F.*, Long, J.*, Cell Discov., 6:66, (2020).

27. “Biochemical  insights into Paf1 complex-induced stimulation of Rad6/Bre1-mediated  monouqbiquitnation”, Chen, F., Liu, B., Guo, L., Ge, X., Feng, W., Li,  D-F*, Zhou, H.*, Long, J.*, Proc. Natl. Acad. Sce. USA, 188(33): e2025291118 (2021).

28. “Crystal  structure of the core module of the yeast Paf1 complex”, Chen, F., Liu,  B., Zeng, J., Guo, L., Ge, X., Feng, W., Li, D-F*, Zhou, H.*, Long, J.*, J Mol Biol, 434(2):167369 (2022).

29.  Phase transition and remodeling complex assembly are important for  SS18-SSX oncogenic synovial sarcomas, Cheng, Y., Shen, Z., Gao, Y.,  Chen, F., Xu, H., Mo, Q., Chu, X., Peng, C-L., Mckenzie, T., Palacios,  B., Hu, J., Zhou, H.*, Long, J.*, Nat. Commun., 13(1): 2724 (2022).

For more information about the published papers, please click the website in: https://www.researchgate.net/profile/Jiafu_Long

1. Biochemistry

2. Structural Biology

3. Protein Function and Techiniques

1. New Century Talent of the Ministry of Education of China (2007)

2. First Prize of the Tianjin Natural Science Award (Ranked Fifth) (2014)

3. Winners in the thirteenth Tianjin Youth Science and Technology Award (2015)