杨雪

课题组聚焦于跨膜信号传导相关重要蛋白的结构与功能的分子机制研究。利用冷冻电镜技术及X-射线晶体学方法解析目标蛋白的高分辨率三维结构，并结合生物化学、细胞生物学、荧光显微成像技术以及电生理学技术，揭示细胞跨膜信号传导的分子机理，为相关疾病的治疗和药物的设计提供有效信息。以第一作者或通讯作者身份在Science Advances、Nature Communications、Cell reports、PNAS、EMBO Journal、PLoS Biology等国际著名刊物上发表学术论文。

2002.9-2006.7 中国农业大学 本科； 2007.9-2012.6 南开大学 生物化学与分子生物学专业 博士。

2012年7月至今 南开大学药物化学生物学国家重点实验室； 2016-2017 加州大学洛杉矶分校 访问学者； 2021年加入南开大学生命科学学院（与药化生国重双聘）。

1. 细胞中关键受体的结构与功能；

2. 细胞跨膜信号传导的分子机制；

3. 细胞受体相关疾病的机制研究。

1.       Y. Ren, Y. Li, Y. Wang, T. Wen, X. Lu, S. Chang, X. Zhang, Y. Shen*, X. Yang*, Cryo-EM structure of the heptameric calcium homeostasis modulator 1 channel, Journal of Biological Chemistry. 2022 Mar 23;101838, online published.

2.       X. Chen, Y. Wang, Y. Li, X. Lu, J. Chen, M. Li, T. Wen, N. Liu, S. Chang, X. Zhang, X. Yang*, Y. Shen*, Cryo-EM structure of the human TACAN in a closed state, Cell Reports. 2022 Mar 1;38(9):110445.

3.       T. Wen, Z. Wang, X. Chen, Y. Ren, X. Lu, Y. Xing, J. Lu, S. Chang, X. Zhang, Y. Shen*, X. Yang*, Structural basis for activation and allosteric modulation of full-length calcium-sensing receptor, Science Advances. 2021 Jun;7(23):eabg1483.

4.       Y. Ren, T. Wen, Z. Xi, S. Li, J. Lu, X. Zhang, X. Yang*, Y. Shen*, Cryo-EM structure of the calcium homeostasis modulator 1 channel, Science Advances. 2020 Jul;6(29):eaba8161.

5.       X. Liu, G. Wu, Y. Yu, X. Chen, R. Ji, J. Lu, X. Li, X. Zhang, X. Yang*, Y. Shen*, Molecular understanding of calcium permeation through the open Orai channel, PLoS Biology. 2019 Apr;17(4):e3000096.

6.       Y. Xing, M. Wang, J. Wang, Z. Nie, G. Wu, X. Yang*, Y. Shen*, Dimerization of MICU Proteins Controls Ca(2+) Influx through the Mitochondrial Ca(2+) Uniporter, Cell Reports. 2019 Jan 29;26(5):1203-1212 e1204.

7.       X. Li, G. Wu, Y. Yang, S. Fu, X. Liu, H. Kang, X. Yang*, X.C. Su*, Y. Shen*, Calmodulin dissociates the STIM1-Orai1 complex and STIM1 oligomers, Nature Communications. 2017 Oct 19;8(1):1042.

8.       L. Wang, X. Yang (co-first), S. Li, Z. Wang, Y. Liu, J. Feng, Y. Zhu, Y. Shen*, Structural and mechanistic insights into MICU1 regulation of mitochondrial calcium uptake, EMBO Journal. 2014 Mar 18;33(6):594-604.

9.       X. Yang, H. Jin, X. Cai, S. Li, Y. Shen*, Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1), Proceedings of the National Academy of Sciences of the United States of America.2012 Apr 10;109(15):5657-5662.

10.    Q. Yan, Y. Shen, X. Yang*, Cryo-EM structure of AMP-PNP-bound human mitochondrial ATP-binding cassette transporter ABCB7, Journal of Structural Biology 2022 Mar;214(1):107832.

11.    Y. Lu, X. Lu, Y. Xu, Y. Ren, Y. Shen, X. Yang*, Expression, purification and microscopic characterization of transmembrane BAX Inhibitor-1 motif containing protein 5, Protein Expression and Purification. 2022 May;193(106045.

12.    S. Li, Y. Ren, X. Lu, Y. Shen, X. Yang*, Cryo-EM structure of human ABCB8 transporter in nucleotide binding state, Biochemistry and Biophysical Research Communications. 2021 Jun 11; 557, 187-191.

13.    Z. Nie, Q. Yan, Y. Shen, X. Yang*, Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein, Protein Expression and Purification. 2021 Jul; 183, 105860.

14.    Y. Zhang, Y. Li, X. Liu, J. Sun, X. Li, J. Lin, X. Yang*, Z. Xi*, Y. Shen*, Molecular architecture of the acetohydroxyacid synthase holoenzyme, Biochemical Journal. 2020 Jul 17; 477(13), 2439-2449.

15.    X. Yang, D.L. Clemens, B.Y. Lee, Y. Cui, Z.H. Zhou*, M.A. Horwitz*, Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo, Structure. 2019 Dec 3;27(12):1811-1819 e1816.

16.    G. Wu, S. Li, G. Zong, X. Liu, S. Fei, L. Shen, X. Guan, X. Yang*, Y. Shen*, Single channel recording of a mitochondrial calcium uniporter, Biochemistry and Biophysical Research Communications. 2018 Jan 29;496(1):127-132.

17.    J. Feng, S. Fu, X. Cao, H. Wu, J. Lu, M. Zeng, L. Liu, X. Yang*, Y. Shen*, Synaptonemal complex protein 2 (SYCP2) mediates the association of the centromere with the synaptonemal complex, Protein & Cell. 2017 Jul;8(7):538-543.

18.    S. Fu, L. Li, H. Kang, X. Yang*, S. Men*, Y. Shen*, Chronic mitochondrial calcium elevation suppresses leaf senescence, Biochemistry and Biophysical Research Communications. 2017 Jun 3;487(3):672-677.

19.    J. Shao, Z. Fu, Y. Ji, X. Guan, S. Guo, Z. Ding, X. Yang*, Y. Cong*, Y. Shen*, Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) forms a Ca(2+)/H(+) antiporter, Scientific Reports. 2016 Sep 27;6(34174.

20.    L. Li, Y. Xing, D. Chang, S. Fang, B. Cui, Q. Li, X. Wang, S. Guo, X. Yang*, S. Men*, Y. Shen*, CaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence, Scientific Reports. 2016 Aug 19;6(31889.

21.    B. Cui, S. Fang, Y. Xing, Y. Shen*, X. Yang*, Crystallographic analysis of the Arabidopsis thaliana BAG5-calmodulin protein complex, Acta Crystallographica Section F: Structural Biology Communications. 2015 Jul;71(Pt 7):870-875.

22.    Z. Wang, X. Yang (co-first), X. Chu, J. Zhang, H. Zhou, Y. Shen*, J. Long*, The structural basis for the oligomerization of the N-terminal domain of SATB1, Nucleic Acids Research. 2012 May;40(9):4193-4202.

23.    Z. Wang, X. Yang (co-first), S. Guo, Y. Yang, X.C. Su, Y. Shen*, J. Long*, Crystal structure of the ubiquitin-like domain-CUT repeat-like tandem of special AT-rich sequence binding protein 1 (SATB1) reveals a coordinating DNA-binding mechanism, Journal of Biological Chemistry. 2014 Oct 3;289(40):27376-27385.

24.    B. Cui, X. Yang (co-first), S. Li, Z. Lin, Z. Wang, C. Dong, Y. Shen*, The inhibitory helix controls the intramolecular conformational switching of the C-terminus of STIM1, PLoS One. 2013 8(9):e74735.

25.    S. Fang, L. Li, B. Cui, S. Men, Y*. Shen*, X. Yang*, Structural insight into plant programmed cell death mediated by BAG proteins in Arabidopsis thaliana, Acta Crystallographica Section D: Structural Biology. 2013 Jun;69(Pt 6):934-945.

26.    C. Dong, X. Yang (co-first), H.F. Hou, Y.Q. Shen*, Y.H. Dong*, Structure of Escherichia coli BamB and its interaction with POTRA domains of BamA, Acta Crystallographica Section D: Structural Biology. 2012 Sep;68(Pt 9):1134-1139.

27.    J. Zhang, X. Yang*, Y. Shen, J. Long, Crystallization and preliminary X-ray data collection of the L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) tripartite complex, Acta Crystallographica Section F: Structural Biology Communications. 2011 Nov 1;67(Pt 11):1443-1447.

28.    X. Yang, X. Xie, L. Chen, H. Zhou, Z. Wang, W. Zhao, R. Tian, R. Zhang, C. Tian*, J. Long, Y*. Shen*, Structural basis for tandem L27 domain-mediated polymerization, FASEB Journal. 2010 Dec;24(12):4806-4815.

29.  X. Yang, Z. Wu, X. Wang, C. Yang, H. Xu, Y. Shen*, Crystal structure of lipoprotein GNA1946 from Neisseria meningitidis, Journal of Structural Biology. 2009 Dec;168(3):437-443.

 (* corresponding author)

2021年1月，入选南开大学百名青年学科带头人培养计划

2021年度天津青科协优秀青年科技工作者