主持或参加科研项目及人才计划项目情况(按领域、时间倒序排序)：
1. 植物种子生理与逆境生理(Plant Seed and Stress Physiology)
(1) 马铃薯种子大小的遗传解析及种质创新(云南省西南联合研究生院科技项目, 2025.1-2027.12)
(2) IPK1在水稻种子发育中的作用及其分子机制(天津市科技计划应用基础研究面上项目, 21JCYBJC00010, 2021.10-2024.9)
(3) 拟南芥E3泛素连接酶ATL5调控种子寿命的作用机制研究(国家自然科学基金面上项目, 32070349, 2021.1-2024.12)
(4) 调控种子寿命的类受体激酶HSL1的功能及其在种质创新上的应用(天津市自然科学基金重点项目, 18JCZDJC33700, 2018.4-2021.3)
(5) 拟南芥类受体激酶HSL1调控种子寿命的功能与作用机制研究(国家自然科学基金面上项目, 31870304, 2019.1-2020.12)
(6) 石漠化环境根际微生物的解磷及与适生砧木的互作机制研究(国家重点研发计划“典型脆弱生态修复与保护研究”重点专项2016年度项目“喀斯特槽谷区土地石漠化过程及综合治理技术研发与示范”之课题任务四“喀斯特槽谷区退化植物群落生态恢复技术与示范”, 2016YFC0502304-03, 2016.7-2020.6, 专题负责人)
(7) 利用基因组编辑技术创制低植酸强耐盐粳稻新种质(天津市自然科学基金重点项目, 17JCZDJC34000, 2017.4-2020.3；天津市水稻产业技术体系创新团队建设项目, ITTRRS2018005, 2018.01-2020.12)
(8) 杜氏盐藻Ds-26-16的功能及广谱耐盐分子机制的研究(国家自然科学基金面上项目, 31570769, 2016.1-2019.12)
(9) 水稻BGlu基因家族成员调控种子寿命的作用与分子机制(国家自然科学基金面上项目, 31571760, 2016.1-2019.12)
(10) 耐盐转录因子的定向改造及耐盐粳稻新种质的创制(天津市自然科学基金重点项目, 14JCZDJC34100, 2014.4-2017.3)
(11) 超耐盐杜氏藻基因的克隆及其在植物耐盐工程中的应用(天津市自然科学基金重点项目, 12JCZDJC22900, 2012.4-2015.3)
(12) 导入细菌三嗪水解酶基因创制无选择标记抗除草剂杂交粳稻亲本新种质(天津市科技支撑计划重点项目, 11ZCGYNC01000, 2011.4-2014.3)
(13) 生育酚和三烯生育酚对水稻种子寿命的影响及作用机制研究(国家自然科学基金面上项目, 31070273, 2011.1-2013.12)
(14) 通过代谢工程获取高α-生育酚优质大豆(天津市科技支撑计划重点项目, 07JCZDJC03800, 2007.4-2010.3)
(15) 植物获得性系统抗性诱导物的筛选(天津市21世纪青年科学基金, 973704311, 1997.4-1999.12)
(16) 毒素Thaxtomin A对种子萌发的影响及其遗传学基础(1997.7-1999.6)
2. 酶工程(Enzymatic Engineering)
(1) 锌指蛋白ZiF引导PTCH1蛋白的穿膜分析及其对牙源性角化囊肿细胞的影响(南开大学中央高校基本科研业务费专项——2019交叉融合科学基金项目, 63191118)
(2) 锌指蛋白-人乳铁蛋白融合蛋白的穿膜效果研究(药物化学生物学国家重点实验室开放研究课题, 201503017, 2015.4-2017.3)
(3) 阿特拉津脱氯酶分子定向进化的研究(国家自然科学基金面上项目, 31070717, 2011.1-2013.12)
(4) 适合高效生产生物燃料的纤维素酶的定向改造(天津市科技支撑计划重点项目国际科技合作专项, 09ZCGHHZ00500, 2009.10-2012.9)
(5) 针对结构未知酶的高效定向进化策略研究(国家自然科学基金面上项目, 30671183, 2007.1-2009.12)
(6) 利用定向进化技术提高糖化酶活性(天津市自然科学基金一般项目, 043606711, 2004.4-2006.12)
(7) c-myc调控基因MIBP1在胶质瘤中的表达和功能的研究(天津市自然科学基金重点项目, 033800911, 2003.4-2006.12)
(8) 利用基因的体外定向进化技术创造高活性GSTZ (留学回国人员科研启动基金, 教外司留2003-406, 2003.7-2006.6; 南开大学2004年度科技创新基金, ZB0001, 2004.1-2005.12)
(9) 花粉与种子发育过程中NAD-IDH基因表达规律(教育部骨干教师基金, 教技司[00]65, 2000.1-2002.12)
(10) Molecular regulation of phytocrome B signal response in potato (Solanum tuberosum L.) tuberization (中国-加拿大“三对三”国际合作项目, 2000.1-2000.12)
(11) 植物雄性不育分子机理的研究(天津市21世纪青年科学基金, 973704111, 1997.3-1999.12)
(12) 花粉与花粉胚发育过程中线粒体基因的表达规律(国家自然科学基金面上项目, 39670080, 南开大学科学发展基金, 1997.1-1999.12)
3. 类胡萝卜素代谢工程(Metabolic Engineering of Carotenoid)
(1) 杜氏盐藻品种改良与番茄红素规模化开发技术研究(海洋公益性行业科研专项经费项目, 200805044, 2008.7-2011.6)
4. 分子标记与QTL定位等
(1) 小麦品质性状QTLs的研究(天津市自然科学基金一般项目, 043612011, 2004.4-2006.12; 南开大学2004年科技创新基金, ZB0001, 2004.1-2005.12)
(2) 黄瓜分子育种技术研究与新品种选育(国家“863”计划优质超高产农作物新品种培育重大专项, 2003AA207110, 2003.7-2005.12)
(3) 利用分子标记技术分析我国栽培大麦资源的遗传多样性(留学回国人员科研启动基金, 教外司留2003-14, 2003.1-2005.12)
(4) 用分子遗传学技术预测黄瓜杂种优势(天津市重大导向性攻关项目, 993122511-2, 2000.1-2002.12)
(5) 不同生态条件下水青冈DNA多态性的研究(杰出人才基金横向合作, 1997.1-1999.12)
代表性研究成果和学术奖励情况(仅展示近5年)：
1) Jiafei Qu, Yongqi Jiang, Shian Wu, Xiwen Chen, Jiemei Zhai, Jing Gao, Yuanzhu Zhou, Yingying Hong*, Jing Shen*, Defu Chen* (2025). GDC-0449 suppresses odontogenic keratocyst aggressiveness in fibroblasts by upregulating SPARC via Hedgehog pathway inhibition. Arch Oral Biol, 179: 106374. doi: 10.1016/j.archoralbio.2025.106374
2) Yuanyuan Lyu, Wenping He, Xinran Zhou, Xue Gao, Mingxia Fan, Defu Chen*, Xiwen Chen (2025). Clathrin light chain 2 is a substrate of ubiquitin ligase ATL5 and negatively regulates seed longevity in Arabidopsis. J Exp Bot, 28: eraf231. doi: 10.1093/jxb/eraf231
3) 蒋永琪, 陈喜文, 申静, 吴世安, 翟洁梅, 李铁军, 高静, 陈德富*, 曲佳菲* (2025). 牙源性角化囊肿PTCH1基因突变的检测及对成纤维细胞迁移的影响. 南开大学学报(自然科学版), 58(1): 61-66
4) 庞晓楠, 李世汉, 贾子瑄, 佟玉玺, 陈喜文, 刘佳*, 陈德富* (2024). Cys2-His2型锌指蛋白安全运输近红外荧光蛋白进入哺乳动物细胞. 南开大学学报(自然科学版), .57(4): 56-60
5) Lina Wang, Jing Cui, Ning Zhang, Xueqin Wang, Jingping Su, María Pilar Vallés, Shian Wu, Wei Yao, Xiwen Chen*, Defu Chen* (2024). OsIPK1 frameshift mutations disturb phosphorus homeostasis and impair starch synthesis during grain filling in rice. Plant Mol Biol, 114: 91. doi: 10.1007/s11103-024-01488-z
6) Siyue Liu, Jinge Xie, Wenqi Luan, Chen Liu, Xiwen Chen*, Defu Chen* (2024). Papiliotrema flavescens, a plant growth-promoting fungus, alters root system architecture and induces systemic resistance through its volatile organic compounds in Arabidopsis. Plant Physiol Biochem, 208: 108474. doi: 10.1016/j.plaphy.2024.108474
7) Wenping He, Run Wang, Qi Zhang, Mingxia Fan, Yuanyuan Lyu, Shuai Chen, Defu Chen*, Xiwen Chen* (2023). E3 ligase ATL5 positively regulates seed longevity by mediating the degradation of ABT1 in Arabidopsis. New Phytol, 239: 1754-1770. doi: 10.1111/nph.19080
8) Siyue Liu, Jinge Xie, Xiwen Chen*, Defu Chen* (2023). Dunaliella Ds-26-16 acts as a global regulator to enhance salt tolerance by coordinating multiple responses in Arabidopsis seedlings. Planta, 257: 110. doi: 10.1007/s00425-023-04149-5
9) Chengliang Wang, Yuanyuan Lyu, Qi Zhang, Hongye Guo, Defu Chen*, Xiwen Chen* (2023). Disruption of BG14 results in enhanced callose deposition in developing seeds and decreases seed longevity and seed dormancy in Arabidopsis. Plant J, 113: 1080-1094. doi: 10.1111/tpj.16102
10) Defu Chen, Hongye Guo, Shuai Chen, Qianying Yue, Pei Wang, Xiwen Chen* (2022). Receptor-like kinase HAESA-like 1 positively regulates seed longevity in Arabidopsis. Planta, 256: 21. doi: 10.1007/s00425-022-03942-y
11) Jinge Xie, Zongqiang Yan, Guifen Wang, Wenzhi Xue, Cong Li, Xiwen Chen*, Defu Chen* (2021). A bacterium isolated from soil in a karst rocky desertification region has efficient phosphate-solubilizing and plant growth-promoting ability. Front Microbiol, 11: 625450. doi: 10.3389/fmicb.2020.625450
12) Xiaofeng Xu#, Jinlong Bei#, Yibo Xuan, Jiayuan Chen, Defu Chen, Stephen C. Barker, Samuel Kelava, Xiaoai Zhang, Shan Gao*, Ze Chen* (2020). Full-length genome sequence of segmented RNA virus from ticks was obtained using small RNA sequencing data. BMC Genomics, 21: 641. doi: 10.1186/s12864-020-07060-5
13) Rui-Juan Ren, Pei Wang, Li-Na Wang, Jing-Ping Su, Lin-Jing Sun, Yue Sun, De-Fu Chen*, Xi-Wen Chen* (2020). Os4BGlu14, a monolignol β-Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice. Plant Mol Biol, 104(4): 513-527. doi: 10.1007/s11103-020-01056-1
14) Chengliang Wang, Dong Li, Pei Wang, Defu Chen*, Xiwen Chen* (2020). Genome-wide analysis of HAESA/HAESA-like kinase family in rice. Amer J Plant Sci, 11: 1254-1269. Doi: 10.4236/ajps.2020.118089
15) Chengliang Wang, Shuai Chen, Yanping Dong, Ruijuan Ren, Defu Chen*, Xiwen Chen* (2020). Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice. New Phytol, 226: 1042-1054. doi: 10.1111/nph.16416
16) Xiufeng Jin#, Zhi Cheng#, Bo Wang, Tung on Yau, Ze Chen, Stephen C. Barker, Defu Chen, Wenjun Bu, Daqing Sun*, Shan Gao* (2020). Precise annotation of human, chimpanzee, rhesus macaque and mouse mitochondrial genomes leads to insight into mitochondrial transcription in mammals. RNA Biol, 17(3): 395-402. doi: 10.1080/15476286.2019.1709746
17) 李青慧, 王丽娜, 郑颖媚, 孙艳香, 陈喜文, 陈德富* (2019). 利用CRISPR/Cas9技术编辑水稻植酸合成酶IPK1基因. 南开大学学报(自然科学版), 52(6): 52-59.
18) Jinge Xie, Wenzhi Xue, Cong Li, Zongqiang Yan, Dong Li, Guoqiang Li, Xiwen Chen*, Defu Chen* (2019). Water-soluble phosphorus contributes significantly to shaping the community structure of rhizospheric bacteria in rocky desertification areas. Sci Rep, 9: 18408. doi: 10.1038/s41598-019-54943-z
19) Xiaonan Pang, Yuxi Tong, Fang Li, Xuan Wei, Xiwen Chen*, Jia Liu*, Defu Chen* (2019). Expression and characterization of human lactoferrin with tandem zinc finger protein in Chlamydomonas reinhardtii. Algal Res, 43: 101635. doi: 10.1016/j.algal.2019.101635
20) Ruijuan Ren, Dong Li, Chunyan Zhen, Defu Chen*, Xiwen Chen* (2019). Specific roles of Os4BGlu10, Os6BGlu24, and Os9BGlu33 in seed germination, root elongation, and drought tolerance in rice. Planta, 249(6): 1851-1861. doi: 10.1007/s00425-019-03125-2
21) Xiaonan Pang, Yuxi Tong, Wenzhi Xue, Yi-feng Yang, Xiwen Chen, Jia Liu*, Defu Chen* (2019). Expression and characterization of recombinant human lactoferrin in edible alga Chlamydomonas reinhardtii. Biosci Biotech Bioch, 83(5): 851-859. doi: 10.1080/09168451.2019.1569498
