环境微生物与微生物催化课题组承担的科研项目如下:
(1)国家重点研发计划“合成生物学”重点专项(2018YFA0900100),真核微生物基因组的人工设计与合成,2019.7-2024.6
(2)天津市自然科学基金一般项目(18JCYBJC24500),采用合成生物学方法在小基因组菌株中构建环境污染物的生物降解途径,2018.4–2021.3
(3)国家自然科学基金面上项目(31570035),恶臭假单胞菌小基因组菌株构建和异源表达多样化有机污染物分解代谢途径,2016.1-2019.12
(4)国家自然科学基金青年项目(31300032),生物安全的多功能农药降解工程菌的构建及其在土壤生物修复中的应用,2014.1–2016.12
(5)天津市自然科学基金青年项目(13JCQNJC09700),门多萨假单胞菌NK-01小基因组菌株构建及其双产物合成,2013.4–2016.3
杨超博士以第一作者或通讯作者发表的SCI论文如下:
Yuting Jiang, Yan Meng, Xinyu Zhang, Ziling Yuan, Haomin Chen, Yujie Liu, Chao Yang,* Ruihua Liu*, 2025. Metabolic engineering of Pseudomonas putida for simultaneous degradation of chlorpyrifos and 2,4-dichlorophenoxyacetic acid and its application in wastewater treatment, ACS EST Engg., under review
Rui Huang, Yujie Liu, Yan Meng, Yuting Jiang, Chao Yang,* Ruihua Liu,* 2025. Production of biological macromolecule ε-poly-L-lysine from rapeseed cake by an engineered Streptomyces albulus, Int. J. Biol. Macromol. major revision
Yaping Chen,‡ Yujie Liu,‡ Yan Meng, Yuting Jiang, Xinyu Zhang, Honglu Liu, Maria A M Reis, Qingsheng Qi,* Chao Yang,* Ruihua Liu,* 2025. Systems metabolic engineering of a genome-reduced Pseudomonas putida for efficient production of polyhydroxyalkanoate from p-coumaric acid, J. Agr. Food Chem. in press
Weini Xiong, Yujie Liu,Yan Meng,Yuting Jiang, Haomin Chen, Ruihua Liu,* Chao Yang,* 2025. Metabolic engineering of Halomonas cupida for efficient mineralization of an organochlorine herbicide 2,4-dichlorophenoxyacetic acid in high saline wastewater, ACS EST Engg. 5: 1289-1297.
Yujie Liu, Yuting Jiang, Yan Meng, Weini Xiong, Ziling Yuan, Ruihua Liu,* Chao Yang,* 2025. Creating a multifunctional degrader for co-mineralization of p-nitrophenol and 1,2-dichloroethane and its application in wastewater bioremediation, J. Hazard. Mater. 488:137417.
Yujie Liu, Weini Xiong, Yuting Jiang, Yan Meng, Wanwan Zhao, Chao Yang,* Ruihua Liu,* 2025. Creating a halotolerant degrader for efficient mineralization of p-nitrophenol substituted organophosphorus pesticides in high saline wastewater. Biotech. Bioeng. 122: 936-947.
Yaping Chen, Yujie Liu, Yan Meng, Yuting Jiang, Weini Xiong, Shufang Wang,* Chao Yang,* Ruihua Liu,* 2024. Elucidating the salt-tolerant mechanism of Halomonas cupida J9 and unsterile ectoine production from lignocellulosic biomass. Microb. Cell Fact. 23: 237.
Weini Xiong, Hongfu Guo, Yujie Liu, Yan Meng, Yuting Jiang, Bingzhi Li,* Ruihua Liu,*Chao Yang,* 2024. Development of halotolerant multi-pesticides degraders for wastewater treatment. Chem. Eng. J. 489:151389.
Siqi Wang, Yujie Liu,Hongfu Guo,Yan Meng,Weini Xiong,Ruihua Liu,*Chao Yang,* 2024. Establishment of low-cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9. Biotechnol. Bioeng. 121 (7): 2106-2120.
Honglu Liu, Yaping Chen, Siqi Wang, Yujie Liu, Wanwan Zhao, Kaiyue Huo, Hongfu Guo, Weini Xiong, Shufang Wang*, Chao Yang*, Ruihua Liu*, 2023. Metabolic engineering of genome-streamlined strain Pseudomonas putida KTU-U27 for medium-chain-length polyhydroxyalkanoate production from xylose and cellobiose. Int. J. Biol. Macromol. 253:126732.
Wanwan Zhao, Weini Xiong, Yujie Liu, Hongfu Guo, Siqi Wang, Yaping Chen, Ruihua Liu*, Bingzhi Li*, Chao Yang*, 2023. Establishment of a halotolerant bioremediation platform from Halomonas cupida using synthetic biology approaches. Chem. Eng. J. 473:145285.
Yu Yang, Yu Deng, Xianghui Shi, Lei Liu, Xiaole Yin, Wanwan Zhao, Shuxian Li, Chao Yang*, Tong Zhang*, 2023. QMRA of beach water by Nanopore sequencing-based viability-metagenomics absolute quantification. Water Res. 235:119858.
Kaiyue Huo, Siqi Wang, Wanwan Zhao, Hongfu Guo, Weini Xiong, Ruihua Liu*, Chao Yang*, 2023. Creating an efficient 1,2-dichloroethane-mineralizing bacterium by a combination of pathway engineering and promoter engineering. Sci. Total Environ. 878:163140.
Yujie Liu, Wanwan Zhao, Siqi Wang, Kaiyue Huo, Yaping Chen, Hongfu Guo, Shufang Wang*, Ruihua Liu*, Chao Yang*, 2022. Unsterile production of a polyhydroxyalkanoate copolymer by Halomonas cupida J9. Int. J. Biol. Macromol. 223:240-251.
Jiayu Zhang,‡ Chao Yang,‡ Jiahui Hu, Yiting Zhang, Yuezheng Lai, Hongri Gong, Fangliang Guo, Xiaoyan Li, Lin Ye, Bing Li*, 2022. Deciphering a novel chloramphenicols resistance mechanism: Oxidative inactivation of the propanediol pharmacophore. Water Res. 225:119127.
Honglu Liu, Yaping Chen, Yiting Zhang, Wanwan Zhao, Hongfu Guo, Siqi Wang, Wenjie Xia, Shufang Wang, Ruihua Liu*, Chao Yang*, 2022. Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering. Int. J. Biol. Macromol. 209:117-124.
Rui Huang, Honglu Liu, Wanwan Zhao, Siqi Wang, Shufang Wang, Jun Cai*, Chao Yang*, 2022. AdpA, a developmental regulator, promotes ε‑poly‑L‑lysine biosynthesis in Streptomyces albulus. Microb. Cell Fact. 21:60.
Kaiyue Huo, Yujie Liu,Rui Huang, Yiting Zhang,Honglu Liu,You Che*, Chao Yang*, 2022. Development of a novel promoter engineering-based strategy for creating an efficient para-nitrophenol-mineralizing bacterium. J. Hazard. Mater. 424:127672.
You Che, Xiaoqing Xu, Yu Yang, Karel Břinda, William Hanage, Chao Yang*, Tong Zhang*, 2022. High-resolution genomic surveillance elucidates a multilayered hierarchical transfer of resistance between WWTPs and human/animal-associated bacteria. Microbiome 10:16.
Yiting Zhang, Honglu Liu, Yujie Liu, Kaiyue Huo, Shufang Wang, Ruihua Liu,* Chao Yang* (2021) A promoter engineering-based strategy enhances polyhydroxyalkanoate production in Pseudomonas putida KT2440. Int. J. Biol. Macromol. 191:608-617
Yuxin Zhao, You Che, Fang Zhang, Jiacheng Wang, Weixia Gao, Tong Zhang, Chao Yang* (2021) Development of an efficient pathway construction strategy for rapid evolution of the biodegradation capacity of Pseudomonas putida KT2440 and its application in bioremediation. Sci. Total Environ. 761:143239
Fang Zhang, Kaiyue Huo, Xingyi Song, Yufen Quan, Shufang Wang, Zhiling Zhang,* Weixia Gao,* Chao Yang* (2020) Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production. Microb. Cell Fact. 19:223
Xu Fan, Yiting Zhang, Fengjie Zhao, Yujie Liu, Yuxin Zhao, Shufang Wang, Ruihua Liu,* Chao Yang* (2020) Genome reduction enhances production of polyhydroxyalkanoate and alginate oligosaccharide in Pseudomonas mendocina. Int. J. Biol. Macromol. 163:2023-2031
Kaiyue Huo, Fengjie Zhao, Fang Zhang, Ruihua Liu,* Chao Yang* (2020) Morphology engineering: a new strategy to construct microbial cell factories.World J. Microb. Biotech. 36:127
Yuxin Zhao, Zhanyi Yao, Deon Ploessl, Saptarshi Ghosh, Marco Monti, Daniel Schindler, Meirong Gao, Patrick Yizhi Cai, Mingqiang Qiao, Chao Yang,* Mingfeng Cao,* Zengyi Shao* (2020) Leveraging the Hermes transposon to accelerate the development of nonconventional yeast-based microbial cell factories. ACS Synth. Biol. 9:1736-1752
Peixin Liang, Yiting Zhang, Bo Xu, Yuxin Zhao, Xiangsheng Liu, Weixia Gao, Ting Ma, Chao Yang,* Shufang Wang,* Ruihua Liu* (2020) Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications. Microb. Cell Fact. 19:70
Fengjie Zhao, Fanyang He, Xiangsheng Liu, Jie Shi, Jingnan Liang, Shufang Wang,* Chao Yang,* Ruihua Liu* (2020) Metabolic engineering of Pseudomonas mendocina NK-01 for enhanced production of medium-chain-length polyhydroxyalkanoates with enriched content of the dominant monomer. Int. J. Biol. Macromol. 154:1596-1605
Xu Fan, Ting Gong, Yunbo Wu, Fengjie Zhao, Mingqiang Qiao, Shufang Wang,* Chao Yang* (2019) Enhanced synthesis of alginate oligosaccharides in Pseudomonas mendocina NK‑01 by overexpressing MreB. 3 Biotech 9:344
Weixia Gao, Yulian He, Fang Zhang, Fengjie Zhao, Chao Huang, Yiting Zhang, Qiang Zhao, Shufang Wang,* Chao Yang* (2019) Metabolic engineering of Bacillus amyloliquefaciens LL3 for enhanced poly-γ-glutamic acid synthesis. Microbial Biotechnol. 12(5):932-945
Yulei Dang, Fengjie Zhao, Xiangsheng Liu, Xu Fan, Rui Huang, Weixia Gao,* Shufang Wang,* Chao Yang* (2019) Enhanced production of antifungal lipopeptide iturin A by Bacillus amyloliquefaciens LL3 through metabolic engineering and culture conditions optimization. Microb. Cell Fact. 18:68
Fengjie Zhao, Ting Gong, Xiangsheng Liu, Xu Fan, Rui Huang, Ting Ma, Shufang Wang,* Weixia Gao,* Chao Yang* (2019) Morphology engineering for enhanced production of medium-chain-length polyhydroxyalkanoates in Pseudomonas mendocina NK-01. Appl. Microbiol. Biotechnol.103(4):1713-1724
Fengjie Zhao, Xiangsheng Liu, Annie Kong, Yuxin Zhao, Xu Fan, Ting Ma, Weixia Gao,* Shufang Wang,* Chao Yang* (2019) Screening of endogenous strong promoters for enhanced production of medium-chain-length polyhydroxyalkanoates in Pseudomonas mendocina NK-01. Sci. Rep. 9:1798
Ting Gong, Xiaoqing Xu, Yulei Dang, Annie Kong, Yunbo Wu, Peixin Liang, Shufang Wang, Huilei Yu, Ping Xu, Chao Yang* (2018) An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates. Sci. Total Environ. 628-629: 1258-1265
Yu Xia,‡Chao Yang,‡ Tong Zhang* (2018) Microbial effects of part-stream low-frequency ultrasonic pretreatment on sludge anaerobic digestion as revealed by high-throughput sequencing-based metagenomics and metatranscriptomics. Biotechnol. Biofuels 11: 47
Chao Yang,‡ Xiaoqing Xu,‡ Yanping Liu,* Hong Jiang, Yunbo Wu, Ping Xu, Ruihua Liu* (2017) Simultaneous hydrolysis of carbaryl and chlorpyrifos by Stenotrophomonas sp. strain YC-1 with surface-displayed carbaryl hydrolase. Sci. Rep. 7: 13391
Ting Gong, Xiaoqing Xu, You Che, Ruihua Liu,* Weixia Gao, Fengjie Zhao, Huilei Yu, Jingnan Liang, Ping Xu, Cunjiang Song,* Chao Yang* (2017) Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane. Sci. Rep. 7: 7064
Chao Yang, Ying Yang, You Che, Yu Xia, Liguan Li, Wenguang Xiong, Tong Zhang* (2017) Bioprospecting for β-lactam resistance genes using a metagenomics-guided strategy. Appl. Microbiol. Biotechnol. 101(15): 6253-6260
You Che, Peixin Liang, Ting Gong, Xiangyu Cao, Ying Zhao, Chao Yang,* Cunjiang Song* (2017) Elucidation of major contributors involved in nitrogen removal and transcription level of nitrogen-cycling genes in activated sludge from WWTPs. Sci. Rep. 7: 44728
Weixia Gao, Fenghong Liu, Wei Zhang, Yufen Quan, Yulei Dang, Jun Feng, Yanyan Gu, Shufang Wang, Cunjiang Song,* Chao Yang* (2017) Mutations in genes encoding antibiotic substances increase the synthesis of poly-γ-glutamic acid in Bacillus amyloliquefaciens LL3. Microbiologyopen 6(1): e00398
Ting Gong, Ruihua Liu, You Che,Xiaoqing Xu, Fengjie Zhao, Huilei Yu, Cunjiang Song,* Yanping Liu,* Chao Yang* (2016) Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos. Microbial Biotechnol. 9(6): 792-800
Chao Yang, Yu Xia, Hong Qu, An-Dong Li, Ruihua Liu, Yubo Wang, Tong Zhang* (2016) Discovery of new cellulases from the metagenome by a metagenomics-guided strategy. Biotechnol. Biofuels 9: 138
Ting Gong, Ruihua Liu,Zhenqiang Zuo, You Che, Huilei Yu, Cunjiang Song,* Chao Yang* (2016) Metabolic engineering of Pseudomonas putida KT2440 for complete mineralization of methyl parathion and γ-hexachlorocyclohexane.ACS Synth. Biol. 5(5): 434-442
Chao Yang, Huilei Yu, Hong Jiang, Chuanling Qiao, Ruihua Liu* (2016) An engineered microorganism can simultaneously detoxify cadmium, chlorpyrifos and γ-hexachlorocyclohexane. J. Basic Microbiol. 56(7): 820-826
Jun Feng, Yanyan Gu, Yufen Quan, Wei Zhang, Mingfeng Cao, Weixia Gao, Cunjiang Song,* Chao Yang,* Shufang Wang* (2015) Recruiting a new strategy to improve levan production in Bacillus amyloliquefaciens. Sci. Rep. 5: 13814
Zhenqiang Zuo, Ting Gong, You Che, Ruihua Liu,* Ping Xu, Hong Jiang, Chuanling Qiao, Cunjiang Song,* Chao Yang* (2015) Engineering Pseudomonas putida KT2440 for simultaneous degradation of organophosphates and pyrethroids and its application in bioremediation of soil. Biodegradation 26(3): 223-233
Yuanyuan Wang, Chi Zhang, Ting Gong, Zhenqiang Zuo, Fengjie Zhao, Xu Fan, Chao Yang,* Cunjiang Song* (2015) An upp-based markerless gene replacement method for genome reduction and metabolic pathway engineering in Pseudomonas mendocina NK-01 and Pseudomonas putida KT2440. J. Microbiol. Methods 113: 27-33
Ruihua Liu, Hong Jiang, Ping Xu, Chuanling Qiao, Qixing Zhou, Chao Yang* (2014) Engineering chlorpyrifos-degrading Stenotrophomonas sp. YC-1 for heavy metal accumulation and enhanced chlorpyrifos degradation. Biodegradation 25(6): 903-910
Ruihua Liu, Zhenqiang Zuo, Yingming Xu, Cunjiang Song, Hong Jiang, Chuanling Qiao, Ping Xu,* Qixing Zhou,* Chao Yang* (2014) Twin-arginine signal peptide of Bacillus subtilis YwbN can direct Tat-dependent secretion of methyl parathion hydrolase. J. Agr. Food Chem. 62(13): 2913-2918
Weitao Geng,‡ Chao Yang,‡ Yanyan Gu, Ruihua Liu, Wenbin Guo, Xiaomeng Wang, Cunjiang Song,* Shufang Wang* (2014) Cloning of ε-poly-L-lysine (ε-PL) synthetase gene from a newly isolated ε-PL-producing Streptomyces albulu NK660 and its heterologous expression in Streptomyces lividans. Microbial Biotechnol. 7(2): 155-164
Chao Yang, Wei Zhang, Ruihua Liu, Chi Zhang, Ting Gong, Qiang Li, Shufang Wang, Cunjiang Song* (2013) Analysis of polyhydroxyalkanoate (PHA) synthase gene and PHA-producing bacteria in activated sludge that produces PHA containing 3-hydroxydodecanoate. FEMS Microbiol. Lett. 346(1): 56-64
Chao Yang, Ruihua Liu, Yulan Yuan, Jianli Liu, Xiangyu Cao,* Chuanling Qiao, Cunjiang Song* (2013) Construction of a green fluorescent protein (GFP)-marked multifunctional pesticide-degrading bacterium for simultaneous degradation of organophosphates and γ-hexachlorocyclohexane. J. Agr. Food Chem. 61(6): 1328-1334
Xiangyu Cao, Chao Yang,* Ruihua Liu, Qiang Li, Wei Zhang, Jianli Liu, Cunjiang Song, Chuanling Qiao, Ashok Mulchandani (2013) Simultaneous degradation of organophosphate and organochlorine pesticides by Sphingobium japonicum UT26 with surface-displayed organophosphorus hydrolase. Biodegradation 24(2): 295-303
Xiangyu Cao, Ruihua Liu, Jianli Liu,* Yapeng Huo, Wei Yang, Meng Zeng, Chao Yang* (2013) A novel polysaccharide from Lentinus edodes mycelia exhibits potential antitumor activity on laryngeal squamous cancer cell line Hep-2. Appl. Biochem. Biotechnol. 171(6): 1444-1453
Ruihua Liu,‡ Chao Yang,‡ Yingming Xu, Ping Xu,* Hong Jiang, Chuanling Qiao* (2013) Development of a whole-cell biocatalyst/biosensor by display of multiple heterologous proteins on the Escherichia coli cell surface for the detoxification and detection of organophosphates. J. Agr. Food Chem. 61(32): 7810-7816
Chao Yang, Wei Zhang, Ruihua Liu, Qiang Li, Baobin Li, Shufang Wang,* Cunjiang Song,* Chuanling Qiao, Ashok Mulchandani (2011) Phylogenetic diversity and metabolic potential of activated sludge microbial communities in full-scale wastewater treatment plants. Environ. Sci. Technol. 45(17): 7408-7415
Yulan Yuan,‡ Chao Yang,‡ Cunjiang Song, Hong Jiang, Ashok Mulchandani,* Chuanling Qiao* (2011) Anchorage of GFP fusion on the cell surface of Pseudomonas putida. Biodegradation 22(1): 51-61
Chao Yang, Cunjiang Song, Roland Freudl, Ashok Mulchandani, Chuanling Qiao* (2010) Twin-arginine translocation of methyl parathion hydrolase in Bacillus subtilis. Environ. Sci. Technol. 44(19): 7607-7612
Chao Yang, Roland Freudl, Chuanling Qiao,* Ashok Mulchandani* (2010) Cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of Escherichia coli by the Tat pathway and ice nucleation protein display system. Appl. Environ. Microbiol. 76(2): 434-440
Chao Yang, Cunjiang Song, Ashok Mulchandani,* Chuanling Qiao* (2010) Genetic engineering of Stenotrophomonas sp. strain YC-1 to possess a broader substrate range for organophosphates. J. Agr. Food Chem. 58(11): 6762-6766
Chao Yang, Roland Freudl, Chuanling Qiao* (2009) Export of methyl parathion hydrolase to the periplasm by the twin-arginine translocation pathway in Escherichia coli. J. Agr. Food Chem. 57(19): 8901-8905
Chao Yang, Qiao Zhao, Zheng Liu, Qiyun Li, Chuanling Qiao,* Ashok Mulchandani,* Wilfred Chen (2008) Cell surface display of functional macromolecule fusions on Escherichia coli for development of an autofluorescent whole-cell biocatalyst. Environ. Sci. Technol. 42(16): 6105-6110
Lin Li,‡ Chao Yang,‡ Wensheng Lan, Shan Xie, Chuanling Qiao,* Jiuxin Liu* (2008) Removal of methyl parathion from artificial off-gas using a bioreactor containing a constructed microbial consortium. Environ. Sci. Technol. 42(6): 2136-2141
Chao Yang, Yaran Zhu, Jijian Yang, Zheng Liu, Chuanling Qiao,* Ashok Mulchandani,* Wilfred Chen (2008) Development of an autofluorescent whole-cell biocatalyst by displaying dual functional moieties on Escherichia coli cell surfaces and construction of a coculture with organophosphate-mineralizing activity. Appl. Environ. Microbiol. 74(24): 7733-7739
Chao Yang, Ning Cai, Ming Dong, Hong Jiang, Jinming Li, Chuanling Qiao,* Ashok Mulchandani,* Wilfred Chen (2008) Surface display of MPH on Pseudomonas putida JS444 using ice nucleation protein and its application in detoxification of organophosphates. Biotechnol. Bioeng. 99(1): 30-37
Hong Jiang,‡ Chao Yang,‡ Hong Qu, Zheng Liu, Q. S. Fu, Chuanling Qiao* (2007) Cloning of a novel aldo-keto reductase gene from Klebsiella sp. strain F51-1-2 and its functional expression in Escherichia coli. Appl. Environ. Microbiol. 73(15): 4959-4965
Chao Yang, Ming Dong, Yulan Yuan, Yao Huang, Xinmin Guo, Chuanling Qiao* (2007) Reductive transformation of parathion and methyl parathion by Bacillus sp. Biotechnol. Lett. 29(3): 487-493
Chao Yang, Na Liu, Xinmin Guo, Chuanling Qiao* (2006) Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil. FEMS Microbiol. Lett. 265(1): 118-125